Microfluidic single-cell cultivation: More than just analysis of single cells?!
Our understanding of microbial organisms is still dominated by an average cell approach in which all cells are seen to behave equally. The complex interplay between environmental dynamics and the subsequent cellular response is still a mystery. Therefore, novel analytical methods need to be developed to understand these phenomena in detail.
In this presentation I will give an introduction into microfluidic single-cell cultivation (MSCC) and its application in biotechnology and bioprocess engineering. MSCC offers precisely controlled external environmental conditions and allows in combination with live-cell imaging single-cell analysis at full spatio-temporal resolution. I will demonstrate the versatility of this technology and how it can be used to get a deeper understanding of microbial physiology on the example of Corynebacterium glutamicum. Latest developments, results as well as technological challenges will be discussed.
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